ABSTRACT
OBJECTIVE: To investigate the relationship between pathogenesis of PBB and the T cell subsets disorders and to evaluate the clinical significance of immunomodulatory therapy to the prognosis of PBB and the prevention of recurrent PBB. METHODS: A total of 127 cases of PBB children treated in the Fourth Hospital of Baotou City from May2015 to May 2018 were selected. Blood samples were collected before treatment,and the levels of CD4,CD8 cells and CD4/CD8 in peripheral blood T cell subsets were detected by flow cytometry. All children with PBB(PBB group)were given oral amoxicillin and clavulanate potassium for 2 to 4 weeks. The subjects were divided into Huaiqihuang group(n=66)and non-Huaiqihuang group(n=66). The Huaiqihuang group was given Huaiqihuang granules based on the anti-infective treatment. In addition,healthy children were enrolled as control group(n=39). T cell subsets of the two groups were reexamined 3 months after treatment. By analyzing the cough symptom scores of all children after 2 weeks,4 weeks and 12 weeks of treatment,the rate of cure and improvement and the recurrence rate after 1 year were evaluated.SPSS16.0 software was used for data processing,and the difference was significant if P0.05). In 2 weeks after treatment,the cough symptom scores of the PBB group began to decrease,and the non-Huaiqihuang group decreased more significantly,with statistically significant difference(P0.05). Follow-up showed that the recurrence rate of Huaiqihuang group was lower than that of non-Huaiqihuang group in 1 year after the end of treatment,and the difference between the two groups was statistically significant(P<0.01). CONCLUSION: Children with PBB have T cell subsets disorder,and the treatment of anti-infection combined with Huaiqihuang granules for at least 4 weeks has a positive effect on PBB prognosis and recurrence prevention.
ABSTRACT
Objective To explore the correlation of the polymorphism of gene IL-13 Intron 3 + 1923and IL-13 and total IgE with pediatric asthma.Methods Polymerase chain reaction-restriction fragment length polymorphisms was used to analyze the polymorphism of gene IL-13 lntron 3 + 1923 in 50 normal children (healthy control group) and 77 asthma children (asthma group); enzyme linked immunosorbent assay was used to measure serum IL-13 and total IgE,and used the SPSS 18.0 software to analyze the serum IL-13 and total IgE in 2 groups.Results 1.The gene type and allele gene frequency distribution of gene IL-13 site Intron 3 + 1923 in asthma group and healthy control group had statistical significance(genotype:x2 =7.239,P ＜ 0.05 ; allele frequency:x2 =6.731,P ＜ 0.01),Allele T had a relationship with asthma.(2) The serum IgE concentration in asthma group was (50.98-± 27.97) mg/L,and in normal control group was (42.85 ± 27.19) mg/L,contrast difference had statistical significant (t =3.067,P ＜ 0.01).(3) The serum IgE in asthma group was (197.13 ±94.7) ng/L and in healthy control group was (159.06 ± 107.65) ng/L,contrast difference had statistical significant (t =2.079,P ＜ 0.05).(4) Different genotype of gene IL-13 Intron 3 + 1923 in asthma group had distinguishing level of IgE:CC was (30.25 ± 19.75) mg/L,CT was (57.50 ± 27.64) mg/L,TT was (65.09 ± 21.14) mg/L,group contrast had significant difference (P ＜ 0.05) ; Different genotype of gene IL-13 Intron 3 + 1923 in asthma group had distinguishing level of IL-13,CC:(105.59 ± 57.32) ng/L,CT:(222.45 ± 74.00) ng/L,TT:(274.38 ±86.12) ng/L,group contrast had significant difference (P＜0.05).Conclusions Polymorphism of gene IL-13 Tntron 3 + 1923 is relevant with children asthma,allele T gene is the correlative gene of children asthma.Allele IL-13 Intron 3 + 1923T has an influence on the level of IL-13,IgE,which makes them higher.Increasing total IL-13 and IgE in serum has a relationship with children asthma.